ABSTRACT
BACKGROUND: Defective dentition is a common oral disease, if it is not treated in time, there will be adverse effects such as tilting of the adjacent teeth and elongation of the jaws, causing occlusal disorder and interference, which will seriously affect the later repair. Especially in the diabetic patients with dentition loss, the impacts of diabetes on the occlusal elongation of the jaw teeth, and how osteonectin changes in this process, are still unclear. OBJECTIVE: To investigate the effect of diabetes on tooth occlusion and elongation in mice by establishing a model of the occlusion of the jaws in diabetic mice. METHODS: A diabetic model was established by intraperitoneal injection of streptozotocin in C57 BL/6J mice (purchased from the Animal Experimental Center of Shanxi Medical University). The mice were intraperitoneally injected with sodium citrate buffer. Thirty mice with successful modeling and control mice were removed, and the three right maxillary molars were removed to establish an experimental model of the extensional movement of the maxillary teeth. After 0, 3, 6, 9 and 12 days, the right jaw was taken. The bone mineral density was measured by micro-CT. The number of osteoclasts was counted by tartrate resistant acid phosphatase staining. The expression level of osteonectin was detected by RT-qPCR. The study was approved by the Ethical Committee of Shanxi Medical University. RESULTS AND CONCLUSION: (1) With the time increasing, the bone mineral density of the right mandible in the two groups was gradually increased. The bone mineral density in the diabetic group was significantly lower than that in the control group at 3, 6, 9 and 12 days after surgery (P < 0.05). (2) With the time increasing, the number of osteoclasts in the right mandible of both groups was gradually increased. The number of osteoclasts in the diabetic group was significantly lower than that in the control group at 3, 6, 9 and 12 days after surgery (P < 0.05). (3) The expression level of osteonectin mRNA in the right mandible of both groups was gradually increased. The expression level of osteonectin mRNA in the diabetic group was significantly higher than that in the control group at 0, 3,6, 9 and 12 days after surgery (P< 0.05). (4) These results indicate that diabetes can reduce the bone construction ability during the extensional movement and promote osteonectin mRNA expression.
ABSTRACT
In the study,nematode-trapping fungus-Arthrobotrys oligospora was firstly cultivated in Sabouraud dextrose broth medium containing 0.05％ of agar,then transferred to the corn meal agar medium.A.oligospora conidiospores was eluted from the media in different time and lyophilized after being counted,then the resuscitation of lyophilized spores was also observed,in oder to evaluate their nematicidal dosage and nematode-trapping efficacy in vitro.The results of the study were as follows:by observing the germination rate,growth rate and nematode-trapping rate of lyophilized spores from A.oligospora.The maximum germination rate of lyophilized A.oligospora conidiospores was 79.5％ on the 4 th day after inoculation,and the average growth rate was 3.4 mm/d;the maximum nematode-trapping rate was 95.8％ on the 7 th day after larvae were added on the media,and the average nematode-trapping rate was 74.0％.Compared with the control groups,the differences were both no significant (P＞0.1)in average growth and nematode-trapping rate.The results show that the freeze-dried preparation materials was accessible and simple,with good resuscitation.After further optimization it will display the prospect of industrialization application.
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Objective: To explore the effect and mechanism of DMDAI-L in inducing the HL-60 cells apoptosis. Methods:Caspase-3 activity in HL-60 cells was measured with the enzymatic visible substrate DEVD-pNA. The fluorescence changes of mito-chondrial membrane potential (△Ψm) in HL-60 cells were investigated with the fluorescent probe JC-1. Results:The caspase-3 activ-ity was significantly increased in HL-60 cells after the DMDAI-L treatment at the concentration of 1. 25, 2. 5, 5, 10 and 20μg·ml-1 for 24h(P<0. 05). DMDAI-L could significantly reduce the mitochondrial membrane potential in HL-60 cells. Conclusion: The mechanism of DMDAI-L in inducing HL-60 cells apoptosis may involve the activation or regulation of caspase-3 activity as well as the reduction of mitochondrial membrane potential in HL-60 cells within certain concentration and time range.
ABSTRACT
The purpose of this study was to clarify the strains of E .granulosus from sheep of Xiwuqi and people of Xil-inhot in Inner Mongolia region and its genotypes .CO1 and ND1 of mitochondria gene were cloned and sequenced ,and then they were analyzed by MegAlign of DNAStar5 .0 .Results showed that the length of CO1 and ND1 gene of E .granulosus ,which were from sheep of Xiwuqi or people of Xilinhot ,were 936 bp and 895 bp ,respectively .The homology of CO1 gene sequences of E .granulosus strains from Xiwuqi and Xinjiang was 99 .3% ,while the homology of the corresponding gene sequences of E .granulosus from man of Xilinhot City and Xinjiang were 98 .6% .ND1 gene of E .granulosus of sheep from Xiwuqi and hu-man from Xilinhot were identical to ND1 gene of G1 type .All these indicated that the homology of E .granulosus from the two regions were high and the genotype were G1 type ,which provided an important basis for the determination of strains ,and it al-so had a great significance to prevent and control the disease .
ABSTRACT
Objective To investigate the telephone information-memory-concentration test (TIMCT) in evaluating the cognitive function of patients with nasopharyngeal carcinoma(NPC)after radiotherapy. Methods The cognitive function were evaluated by TIMCT and mini mental state examination (MMSE) in 2 weeks for 30 normal persons and 90 NPC patients. And the 90 NPC patients were divided into the 3 months, 2 years and 5 years after radiotherapy groups. All patients were carried out firstly face to face interview and telephone interview 1 time after 2 weeks. Results The correlation coefficient of all groups between TIMCT(telephone) and TIMCT (face to face) were bigger (R = 0.850) when MMSE wasn't control variable. And the correlation coefficients between TIMCT (telephone) and TIMCT (face to face) were lower (R = 0.366) when MMSE was control variable. As for examining time was classification factor, TIMCT (telephone) and TIMCT (face to face) were analyzed by partial correlation analysis. Only normal group was correlated with group of 3 months after radiotherapy and group of 2 years after radiotherapy wasn't correlated with group of 5 years after radiotherapy (R = 0.447,0.970,0.200 and 0.062). In addition, the difference plot of TIMCT(telephone) and TIMCT (face to face) indicated that telephone was consistent with face to face interview (MMSE≥28). Both telephone and face to face interview reflected the cognitive function downtrend of rescareh objects. Conclusions TIMCT (telephone), TIMCT(face to face) and MMSE (face to face) can reflect cognitive function downterend of patients with NPC after radiotherapy. But TIMCT(telephone) used in clinical screening cognitive function impairment of patients with NPC after radiotherapy should be improved further.